Anti-Astrovirus 2 [PL-2]
Catalogue Number: AB03163-10.3-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Human astrovirus 2; HAstV-2; Capsid polyprotein VP90; Capsid protein; HAstV-2 capsid spike protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | PL-2 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was isolated by immunizing BALB/c mice with H-Ast-2 virus. |
| Application: | ELISA, IP, NT, SPR, Crstapy |
Additional Text
Purification
Purified
Uniprot ID
Q82446
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The original antibody (IgG2a) was used for immunofluorescence staining of H-Ast2-infected LLCMK2 cells. The antibody neutralizes HAstV serotype 2 (Sanchez-Fauquier et al., 1994; PMID: 7514320). The original antibody (IgG2a) and the scFv fragment bound to wheat germ extract containing recombinant HAstV-2 capsid protein, as confirmed by ELISA analysis. The crystal structure of the Fab fragment was determined. The scFv fragment was used to immunoprecipitate HAstV-2 capsid protein. This antibody could potentially be developed as a therapeutic and diagnostic agent (Bogdanoff et al., 2016; PMID: 27213181). The specificity of the antibody and scFv fragment for the HAstV-2 CP spike were confirmed by ELISA. The binding affinity of the Fab and scFv fragments towards the spike protein was found to be nearly identical (KD of 1.8 nM) using surface plasmon resonance. The structure of the HAstV capsid spike domain bound to the neutralizing scFv fragment was determined. The antibody blocked the attachment of recombinant HAstV-2 CP spike to Caco-2 cells supporting the hypothesis the antibody neutralizes HAstV by blocking virus attachment to cells (Bogdanoff et al., 2016; PMID: 27807234).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
AB03163-10.3-BT Datasheet
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