Anti-CD3 [CD3B219]
Catalogue Number: AB03166-1.1-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; SP34-2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | CD3B219 |
| Isotype: | IgG1 |
| Immunogen: | |
| Application: | FC, InVitroA |
Additional Text
Gene Name
CD3E
Gene ID
916
Uniprot ID
P07766
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The antibody was used to construct different CD33 xCD3 bispecific antibodies (IgG4). In vitro T cell mediated cytotoxicity assays were performed using the bispecific antibody and the cytotoxicity was measured by flow cytometry. Ex vivo cytotoxicity assay showed the paired antibody promoted a dose-dependent reduction of total cytotoxicity that correlated with T cell activation after 48 hours (US20210040209A1). The antibody is considered a high affinity binder (Kd= 8mM) (US20210214440A1). The CD33xCD3 bispecific antibodies specifically induce cell cytotoxicity of MOLM-13 cells and CD33+ monocytes at 48 hr (US20200048349A1).
Short Description
This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
AB03166-1.1 Datasheet
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