Anti-Kit [79D]
Catalogue Number: AB03168-10.3-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Mast/stem cell growth factor receptor Kit (EC:2.7.10.1); SCFR; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD117 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | 79D |
| Isotype: | IgG1 |
| Immunogen: | Phage pools consisting of a phage-displayed synthetic antibody library (library F) were cycled through five rounds of selections by using KITD4-5. |
| Application: | IP, WB, Inh, SPR, Crstapy |
Additional Text
Gene ID
3815
Gene Name
KIT
Uniprot ID
P10721
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The crystal structure of the Fab in complex with KIT D4D5 fragment was determined. Surface plasmon resonance analysis was employed to quantitatively characterize the kinetics and dissociation constants of the Fab (Kd = 6.4 pM). 3T3 cells expressing WT KIT receptor were incubated with the IgG and Fab versions of the antibody following stimulation with SCF. Lysates of unstimulated or SCF-stimulated cells were subjected to immunoprecipitation with the IgG and Fab followed by SDS/PAGE and immunoblotting with IgG and Fab. SCF-stimulated autophosphorylation of KIT was strongly inhibited upon binding of both fragments (5 nM for Fab and 1 nM for IgG). The IgG and Fab fragments inhibited the proliferation of KIT-Dependent Ba/F3 cells. The Fab fragment showed inhibition of cell growth at 5 nM, while the IgG fragment at 10 nM (Reshetnyak et al. 2013; PMID: 24127596).
AB03168-10.3-BT Datasheet
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