Anti-E protein [T025]
Catalogue Number: AB03172-10.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | tick-borne encephalitis virus; TBEV; envelope domain III; EDIII; envelope protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Human |
| Clone: | T025 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was isolated from memory B cells of a single human donor who had recovered from natural TBEV infection during outbreaks in the Czech Republic (specifically in/around Ceske Budejovice) in 2011 or 2018. |
| Application: | ELISA, IF, NT, InVivoA, Crstion |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Gene Name
POLY
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Gene ID
1489719
UniProt ID
P14336
Short Description
This reformatted human antibody was made using the variable domain sequences of the original Human IgG format for improved compatibility with existing reagents assays and techniques.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody showed neutralizing activity against TBEV RVPs (IC50 <1 ng/ml) and authentic TBEV Hypr strain (a TBEVWE isolate; IC50=67.2 ng/ml). Immunofluorescence was performed on PS cells using this antibody. Prophylaxis and therapeutic experiments in BALB/c mice infected with TBEV Hypr showed the antibody was efficacious in prevention (even at low doses ~0.005 mg/kg) and early treatment of the infection (up to 3 days post-infection). The crystal structure of the Fab fragment in complex with the EDIII domains from all three TBEV subtypes was determined (Agudelo et al. 2021; PMID: 33831141).
AB03172-10.0 Datasheet
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