Anti-Envelope protein domain III [POWV-80]
Catalogue Number: AB03175-4.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Core protein; Envelope protein E; Matrix protein; Peptide pr; Protein pr; Small envelope protein M; POWV |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | POWV-80 |
| Isotype: | IgG2c |
| Immunogen: | The original antibody was originated by immunizing C57BL/6J mice with POWV-SPO. |
| Application: | ELISA, FC, NT, Crstapy |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
UniProt ID
Q91LY1
Application Notes
The specificity of the antibody (IgG2c) was determined by ELISA. The antibody was used for flow cytometric analysis of HEK-293T cells. The antibody showed neutralizing activity on Vero cell incubated with POWV MA51240. The antibody showed neutralizing activity also against TBEV and LGTV (EC50 respectively 8.4 and 103 ng/ml). The binding affinity of the antibody to POWV E was measured using biolayer interferometry (BLI), showing the antibody binds POWV E with an affinity (KD) of ~30 nM. The crystal structure of the Fab fragment in complex with POWV DIII was determined. The antibody might have therapeutic potential (VanBlargan et al., 2021; PMID: 33831142).
AB03175-4.0-BT Datasheet
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