Anti-Modified cytokeratin [COU-1]
Catalogue Number: AB03187-1.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | KRT8; CYK8; Keratin type II cytoskeletal 8; Cytokeratin-8; Type-II keratin Kb8; KRT18; CYK18; Keratin type I cytoskeletal 18; Cytokeratin-18; Cell proliferation-inducing gene 46 protein; COU1; B9165; ECACC 87040201 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | COU-1 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by fusing the human lymphoblastoid cell line WI-L2-729-HF2 with lymphocytes obtained from mesenteric lymph nodes from a colon cancer patient. |
| Application: | ELISA, ICC, WB, IHC, Img |
Additional Text
Purification
Purified
Uniprot ID
P05783, P05787
Antibody Clonality
Recombinant Monoclonal
Gene ID
3856; 3875
Gene Name
KRT18, KRT8
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody reacted with a panel of colon cancer cell lines and melanoma cell lines in a immunocytochemical assay. This antibody strongly reacted with antigens expressed on autologous and allogeneic colorectal cancers in an immunohistochemical analysis of formalin-fixed paraffin-embedded tissues (PMID: 3699931). Electron microscopy of colonic adenocarcinoma cells, intact tumor and colonic epithelium by the immunogold technique demonstrated that the C-OU1 antibody reacted with a molecule associated with areas of disruption of the intermediate filaments in the cytoplasm of the tumor cells (PMID: 1607210). This antibody can also recognize modified cytokeratin from tumor extracts in a western blot (PMID: 1607210). This binding of this antibody to different recombinant heterotypic K8/K18 complexes was determined by ELISA. This antibody cannot bind K8(1-65)/intact K18 and intact K8(1-85)/intact K18 complexes in an ELISA (US20050048070). 131 Iodine labeled COU-1 antibody was also used in the detection and localization of tumors using immunoscintigraphy (PMID: 8306271; 8261404). The binding of this antibody to modified cytokeratin expressed on the surface of carcinoma cells causes their endocytosis, demonstrating that modified cytokeratin may serve as potential targets for delivery of immunoconjugates into cancerous cells (PMID: 9223323).
Short Description
This chimeric mouse antibody was made using the variable domain sequences of the original Human IgM format for improved compatibility with existing reagents assays and techniques.
AB03187-1.1-BT Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations