Anti-Modified cytokeratin [COU-1]

Catalogue Number: AB03187-10.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	KRT8; CYK8; Keratin type II cytoskeletal 8; Cytokeratin-8; Type-II keratin Kb8; KRT18; CYK18; Keratin type I cytoskeletal 18; Cytokeratin-18; Cell proliferation-inducing gene 46 protein; COU1; B9165; ECACC 87040201
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Human
Clone:	COU-1
Isotype:	IgG1
Immunogen:	The original antibody was generated by fusing the human lymphoblastoid cell line WI-L2-729-HF2 with lymphocytes obtained from mesenteric lymph nodes from a colon cancer patient.
Application:	ELISA, ICC, WB, IHC, Img

Overview
Datasheets

Additional Text

Purification

Purified

Uniprot ID

P05783, P05787

Antibody Clonality

Recombinant Monoclonal

Gene ID

3856; 3875

Gene Name

KRT18, KRT8

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody reacted with a panel of colon cancer cell lines and melanoma cell lines in a immunocytochemical assay. This antibody strongly reacted with antigens expressed on autologous and allogeneic colorectal cancers in an immunohistochemical analysis of formalin-fixed paraffin-embedded tissues (PMID: 3699931). Electron microscopy of colonic adenocarcinoma cells, intact tumor and colonic epithelium by the immunogold technique demonstrated that the C-OU1 antibody reacted with a molecule associated with areas of disruption of the intermediate filaments in the cytoplasm of the tumor cells (PMID: 1607210). This antibody can also recognize modified cytokeratin from tumor extracts in a western blot (PMID: 1607210). This binding of this antibody to different recombinant heterotypic K8/K18 complexes was determined by ELISA. This antibody cannot bind K8(1-65)/intact K18 and intact K8(1-85)/intact K18 complexes in an ELISA (US20050048070). 131 Iodine labeled COU-1 antibody was also used in the detection and localization of tumors using immunoscintigraphy (PMID: 8306271; 8261404). The binding of this antibody to modified cytokeratin expressed on the surface of carcinoma cells causes their endocytosis, demonstrating that modified cytokeratin may serve as potential targets for delivery of immunoconjugates into cancerous cells (PMID: 9223323).

Short Description

This reformatted human antibody was made using the variable domain sequences of the original Human IgM format for improved compatibility with existing reagents assays and techniques.

AB03187-10.0 Datasheet

Price

£390.00

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