Anti-E Cadherin [19A11]

Catalogue Number: AB03204-10.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	CD324; Cadherin-1; Cadherin E; E-Cadherin; epithelial cadherin; CAM 120/80; Uvomorulin
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Human
Clone:	19A11
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing mice with purified ECad1-5 protein.
Application:	ELISA, FC, WB, Agon

Overview
Datasheets

Additional Text

Gene Name

CDH1

Gene ID

999

Uniprot ID

P12830

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The initial screeding of E-Cadherin-positive hybridoma supernatants for binding to live Colo 205 cells was done using an ELISA. Activating antibody 19A11 stimulated the adhesion of Colo 205 cells to pure E-cadherin substrate (PMID: 22513089). This antibody is also capable of recognizing denature human E-cadherin in a western blot. This antibody also inhibited the formation of epithelial tubules, causing the cells to remain as round epithelial cysts. This antibody also enhanced the adhesion of HGF-treated MDCK cells to E-cadherin when analyzed with the flow adhesion assay (PMID: 22513089). This antibody slowed the rate of wound closure in both normal culture medium and HGF-treated cultures (PMID: 22513089). This antibody is an E-cadherin activating antibody which stimulates the apoptosis of circulating tumor cells (PMID: 32127478). This antibody was used in determination of expression of E-cadherin on low-adherent Colo205 cells using flow cytometry (PMID: 27582386).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

AB03204-10.0 Datasheet

Price

£390.00

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