Anti-Prion protein [D18]

Catalogue Number: AB03218-23.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	PrPC; ASCR; PrP27-30; PrP33-35C; CD230
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Rabbit
Clone:	D18
Isotype:	IgG
Immunogen:	PrP deficient (Prnp0/0) mice were immunized with disaggregated PrP 27-30 incorporated into liposomes. The original antibody was generated by panning by panning against dispersed SHaPrP 27-30.
Application:	ELISA, FC, IP, WB, Inh, InVivoA, SPR

Overview
Datasheets

Additional Text

Gene Name

PRNP

Uniprot ID

P04156

Gene ID

5621

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The specificity of the antibody to recombinant SHaPrP(90-231) and with PrP 27-30 rods was confirmed by ELISA. The epitope mapping of this antibody was done using a paptide-based ELISA. The Fab fragment reacted with cell surface MoPrPc as showed by flow cytometry. The antibody efficiently immunoprecipitated SHaPrPC from transfected CHO cells (Williamson et al. 1998; PMID: 9765500). The ability of of the Fab fragment to inhibit prion propagation in a cultured mouse neuroblastoma cell line infected with PrPSc (ScN2a) was tested. The Fab fragment was found to abolish prion replication and to clear pre-exiting PrPSc, eliminating 50% of PrPSc from the cells within about 24 hours. Therefore, the antibody showed potential as a therapeutic agent. This antibody was used for detection of PrP with the surface of ScN2a cells by flow cytometry (Peretz et al., 2001; PMID: 11507642). The scFv version of the antibody was constructed and the specificity tested by ELISA. The binding affinity of the scFv fragment to PcP was measured by surface plasmon resonance (Kd= 10.1 nM). The scFv antibody gene was delivered to the brain by a recombinant adeno-associated virus, where it delayed the progression of the disease in a mouse model of TSE (Wuertzer et al., 2008; PMID: 18180775). The Fab fragment was conjugated to UltreSmall gold particles and used for immunolabelling for PrPC localization in mouse hippocampus (Mironov et al., 2003; PMID: 12904479). Immunofluorescence was performed on RK13 cells using this antibody (Spagnolli et al., 2021; PMID: 33437023).

AB03218-23.0 Datasheet

Price

£390.00

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