Anti-Lysozyme [F9.13.7]
Catalogue Number: AB03220-1.1-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Lysozyme C; 1,4-beta-N-acetylmuramidase C; GEL; hen egg-white lysozyme |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Mouse |
| Clone: | F9.13.7 |
| Isotype: | IgG1 |
| Immunogen: | The antibody was raised against hen egg-white lysozyme. |
| Application: | ELISA, Crstapy |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Uniprot ID
P00704
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis (Lescar et al., 1993; PMID: 7680133). The structure of the Fab fragment in complex with Guinea-Fowl Lisozyme was determined (Lescar et al., 1995; PMID: 7629116). Homogeneous kinetics of Fab and Fv fragments were studied and the KD values were 0.57 +/- 0.05 nM for scFv and 1.6 +/- 0.2 nM for Fab. Since the clone and HyHEL-10 recognized a common epitope, equilibria and kinetics of the two clones were evaluated. Results showed that the clone had greater specificity than HyHEL-10 despite its lower affinity (Pons et al., 2002; PMID: 12237453). The ability of the antibody to modulate in vitro the presentation of hen egg-white lysozyme (HEL) to specific T cells was evaluated, results showed the antibody was able to enhance the HEL presentation to L3E10 T cells (Guermonprez et al., 1999).
AB03220-1.1 Datasheet
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