Anti-Manganese transport protein C [305-78-7]

Catalogue Number: AB03221-23.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Rabbit
Clone:	305-78-7
Isotype:	IgG
Immunogen:	The original antibody was generated by immunizing mice with purified recombinant MntC.
Application:	ELISA, InVitroA, InVivoA, ITC, Crstapy

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Specificity

MntC; ABC transporter substrate-binding protein; Manganese ABC transporter substrate-binding lipoprotein; Manganese ABC transporter substrate-binding protein; Manganese-binding lipoprotein mntA; Manganese-binding protein; Metal ABC transporter substrate-binding protein; Periplasmic solute binding protein

UniProt ID

W8TNQ9

Application Notes

The antibody showed reduce S. aureus burden in an infant rat passive protection model and induced neutrophil respiratory burst activity against both Staphyloccocus aureus and Staphyloccocus epidermidis (Anderson et al., 2012; PMID: 22474033). The structure of the antibody in complex with MntC was determined. Epitope mapping was carried out using Hydrogen-Deuterium Exchange Mass Spectrometry, the results were further validated using X-ray crystallography, site-directed mutagenesis and functional studies. The binding of the antibody to MntC was tested using ITC and ELISA. ITC experiments showed that binding of the wild type MntC to the antibody was stoichiometric, the apparent Kd was 1.2 nM, and the binding enthalpy was -19.9 kcal/mol. The specificity of the antibody was confirmed by ELISA analysis, with Kd= 1.3 nM. (Gribenko et al., 2016; PMID: 27689696)

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

AB03221-23.0 Datasheet

Price

£390.00

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