Catalogue Number: AB03227-1.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | H4K5acK8ac; Histone H4 protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | 1A9D7 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing mice with Histone H4 protein with site-specific acetylations at K5 and K8. |
| Application: | ELISA, WB, Crstion |
P62805
Purified
121504; 554313; 8294; 8359; 8360; 8361; 8362; 8363; 8364; 8365; 8366; 8367; 8368; 8370
Recombinant Monoclonal
H4-16, H4C1, H4C11, H4C12, H4C13, H4C14, H4C15, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
The specificity of the original format of the antibody for histone H4 proteins containing di-acetylation of K5acK8ac was confirmed by ELISA analysis. The antibody detected the histone H4 proteins containing at least the di-acetylation of K5acK8ac western blot analysis. The crystal structure of the complex of di-acetylated histone H4 and 1A9D7 Fab fragment was determined (Handoko et al., 2018; PMID: 30080437).
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format for improved compatibility with existing reagents assays and techniques.