Anti-FAP [73.3]
Catalogue Number: AB03229-1.1-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Mouse |
| Clone: | 73.3 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing FAP-null mice with FAP-expressing 3T3 cells. |
| Application: | FC, IP, WB, InVitroA, InVivoA |
Additional Text
Gene Name
FAP
Uniprot ID
P97321
Gene ID
14089
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity
Fibroblast activation protein; Fibroblast activation protein-α; Prolyl endopeptidase FAP; Dipeptidyl peptidase FAP; Fibroblast activation protein alpha; FAPalpha1; Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme;Serine integral membrane protease; SIMP; Surface-expressed protease; Seprase
Application Notes
A second-generation retroviral CAR, consisting of the scFv fragment of the antibody, with CD8 stalk, human CD3ζ and 4-1BB domains, targeting mouse FAP was developed. The Fap-CAR-T cells killed FAP-expressing 3T3 target cells specifically. The FAP-CAR-T cells were tested in vivo in three different established models, showing that the growth of tumors was significantly reduced by 35-50% following treatment. Further, the anti-FAP-CAR T cells demonstrated enhanced antitumor response when combined with a tumor vaccine. No significant toxicity was observed in the treated mice. (Wang et al., 2014; PMID: 24778279) FAP+ cells were identified using flow cytometry of lung tissue using the antibody (Kimura et al., 2019; PMID: 31188013). This antibody was used for detection of murine FAP expression on HT1080 cells by flow cytometry (de Sostoa et al. 2019, PMID: 30683154). Immunoprecipitation of FAP was performed on lung humogenate using this antibody. The antibody detected FAP by western blot analysis (Fan et al., 2016; PMID: 26663085).
AB03229-1.1 Datasheet
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