Anti-CTLA-4 [5E3]
Catalogue Number: AB03236-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CD152 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | 5E3 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with CTLA-4 protein. |
| Application: | ELISA, InVivoA |
Additional Text
Uniprot ID
P16410
Gene ID
1493
Gene Name
CTLA4
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The specificity of the antibody was confirmed by ELISA analysis. The kinetic parameters of human CTLA-4 protein binding to the antibody were measured and they resulted to be at the sub-nm level. The antitumor activity of the humanized antibody was demonstrated in vivo in animal models. It was proved that the antibody inhibited the tumor in a dose-dependent manner. Mouse PD-1 antibody was combined with the antibody and used to evaluate the toxicity to mice; these experiments compared with the use of Ipilimumab in combination with PD-1 antibody. Results showed that after 5E3 antibody was used in combination with PD-1 antibody, the immunity to the body and the toxicity of heart, liver and lung are significantly lower than the toxicity of Ipilimumab in combination with PD-1 antibody (CN110343180).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
AB03236-23.0-BT Datasheet
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