Anti-Lipoprotein lipase [5D2]

Catalogue Number: AB03241-6.1-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	LPL; Phospholipase A1; EC 3.1.1.34
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Rat
Clone:	5D2
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing mice with bovine LPL.
Application:	ELISA, ICC, IF, WB, NT, SPR, Crstapy

Overview
Datasheets

Additional Text

Gene Name

LPL

Uniprot ID

P06858

Gene ID

4023

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

LPL was immunoprecipitated from postheparin plasma using this antibody. The antibody was used both to capture LPL and to detect the bound LPL in a sandwich ELISA, indicating the epitope to be present in duplicate. In another sandwich ELISA analysis, the antibody was used to detect the LPL antigen (Peterson et al., 1992, PMID: 1279089). The antibody detected LPL from human, chicken and guinea pig by western blot analysis. The specificity of the original format of the antibody was confirmed by ELISA analysis (Chang et al., 1998; PMID: 9831623). Pretreatment of LPL with the antibody totally suppressed LPL-induced monocyte adhesion to endothelial cells (Mamputu et al., 1997; PMID: 9323582). Further, LDL was immunoneutralized with the antibody, totally suppressing LPL-induced VSMC proliferation (Mamputu et al., 2000; PMID: 11031206). Immunofluorescence was performed on LPL expressed on CHO-K1 cells using this antibody (Voss et al., 2011; PMID: 21518912). The binding affinity of the antibody to a synthetic LPL peptide containing the Trp-rich loop of human, mouse, bovine, rat and chicken, was measured by surface plasmon resonance.The binding affinity of the antibody to the human, bovine and chicken LPL peptides was high (KD of 0.19, 0.78 nM and 0.34 nM, respectively), the binding affinity for the rat LPL peptide was reduced (Kd= 2.99nM), and no binding to the Trp-rich peptide from mouse LPL was detected. The antibody detected LPL by western blot analysis. By immunocytochemistry, the antibody bound avidly to the wildtype human LPL on CHO cells. The crystal structure of the Fab version of the antibody was solved (Luz et al., 2020; PMID: 32690595).

Short Description

This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

AB03241-6.1-BT Datasheet

Price

£1,655.00

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