Anti-N type Glycophorin A [NNA7]
Catalogue Number: AB03281-1.1-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD235a; Glycophorin-A; GYPA; GPA; PAS-2; N sialoglycoprotein; Sialoglycoprotein alpha; Glycophorin A N antigen; MNS blood group antigen; glycopeptide N blood-group antigen; red blood cell antigen; N-type glycophorin A; N72 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Mouse |
| Clone: | NNA7 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was isolated from light-chain cDNA libraries constructed from mice immunized with N-type glycophorin A. These were inserted into a recombinant pComb3H vector containing the N92 Fd fragment. The N92 Fd fragment: light-chain libraries were panned on N-type glycophorin A or NN red cells. |
| Application: | ELISA, IF, WB, Aggl |
Additional Text
Gene Name
GYPA
Gene ID
2993
Uniprot ID
P02724
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody is a synthetic clone derives from the parental clone N72 Fab. This antibody is capable of reacting with N-type glycophorin A and NN red cells in an ELISA and an agglutination assay (PMID: 10220261). This antibody is capable of detecting glycophorin A of N-type and glycophorin B in a western blot assay (PMID: 19833240; 9574545). This antibody was also used in the immunofluorescence staining of transfected CHO cells expressing recombinant N-type GPA (PMID: 14962308).
Short Description
This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.
AB03281-1.1 Datasheet
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