Anti-Collagen type II [CIIC1]

Catalogue Number: AB03291-10.0-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	CII; Alpha-1 type II collagen; Collagen alpha-1(II) chain
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Human
Clone:	CIIC1
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing DBA/1 mice with chick sternal type II collagen.
Application:	ELISA, WB, IHC, InVitroA, InVivoA, Crstion

Overview
Datasheets

Additional Text

Gene Name

COL2A1

Uniprot ID

P02458

Gene ID

1280

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

This anti-collagen type II antibody CIIC1 is an arthritogenic antibody, which induces arthritis in mice (Holmdahl et al., 1986; PMID: 2421741 and Nandakumar et al., 2003; PMID: 12884302). The antibody specificity towards collagen type II was assayed in an ELISA. The antibody was selected for in vivo injection experiments in naive DBA/1 male mice, at no time was any arthritis detected macroscopically after treatment with 0.5, 2 or 5 mg of antibody per animal, however development of synovitis was noticed (Holmdahl et al., 1986; PMID: 2421741). The structure of the Fab fragment was determined by X-ray crystallography. The specificity of the full length CIIC1 antibody and the FV fragment was confirmed by ELISA analysis. Further investigation by using both the full length antibody and a single chain Fv fragment in ELISA experiments revealed that the antibody bound also to other IgG1, IgG2a and IgG2b anti-collagen antibodies (Uysal et al., 2008; PMID: 18241923). The antibody detected collagene type II by western blot analysis (Burkhardt et al., 2005; PMID: 15832289). In vitro studies demonstrated that the antibody together with M2139 antibody suppressed the self-assembly of CII into fibrils (Croxford et al., 2010; PMID: 20662051). This antibody was used for immunohistochemistry on cartilaginous tissue (Vickers et al., 2010; PMID: 20225321).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

AB03291-10.0-BT Datasheet

Price

£1,655.00

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