Anti-Galectin-2 [FF06]

Catalogue Number: AB03293-23.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Gal-2; Beta-galactoside-binding lectin L-14-II; HL14; Lactose-binding lectin 2; S-Lac lectin 2
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Rabbit
Clone:	FF06
Isotype:	IgG
Immunogen:	The original antibody was isolated from the antibody library after two rounds of panning against human galectin-2.
Application:	ELISA, IF, InVivoA

Overview
Datasheets

Additional Text

Gene Name

LGALS2

Gene ID

3957

Uniprot ID

P05162

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The scFv fragment was able to bind the recombinant human galectin-2 protein in ELISA experiments. Immunofluorescence analysis on the colon biopsy samples from a healthy donor was performed. The scFv fragment showed significant staining surrounding the crypts of the intestinal tissue. Immunofluorescence analysis on the human colorectal adenocarcinoma cell line LS174T was performed in order to verify the ability of the scFv fragment to recognize natively expressed galectin-2 antigen. The ScFv fragment showed strong cytoplasmic staining. Immunofluorescence analysis on human biopsy samples of Ulcerative Colitis was performed. A stronger intensity signal in the colonic crypts region can be detected in the sections stained with the scFv fragment when compared to the signal observed with an irrelevant antibody. The autoradiography on the intestinal tract of a mouse having colitis has been performed in order to verify the ability of the scFv fragment to recognize the galectin-2 antigen in vivo. A stronger intensity signal could be detected in the colon of the mouse injected with the scFv fragment when compared to the signal in a control mouse injected with an irrelevant antibody (US20190309060).

Short Description

This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.

AB03293-23.0 Datasheet

Price

£390.00

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