Anti-Ricin [A9]
Catalogue Number: AB03296-23.159-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | rRNA N-glycosidase; Ricin A chain (EC:3.2.2.22); Ricin B chain |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.2 mg |
| Host name: | Rabbit |
| Clone: | A9 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing two alpacas with a ricin toxoid. A VHH phage-displayed library was constructed and the antibody was identified in a panning directly on plate bound RTA. |
| Application: | ELISA, NT, Crstapy |
Additional Text
Purification
Purified
Gene ID
8261245
Gene Name
LOC8261245
Uniprot ID
P02879
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The VHH antibody was specific for RTA as confirmed by competition ELISA. The crystal structure of ricin catalytic subunit in complex with the VHH antibody was determined. The antibody had relatively weak toxin-neutralizing activity (IC50 ~750 nM), as shown by Vero cell cytotoxicity assays. The antibody binding affinity (KD) for ricin holotoxin (0.08 nM). The binding affinity, and, toxin-neutralizing activity of the antibody was mediated by CDR2 containing five consecutive Gly residues that interact with α-helix B. In order to prove this, a variant of the antibody lacking Gly AC151residue 59 (A9Δ59) was generated. Binding studies and toxinneutralizing assays confirmed that the removal of a single glycine residue from A9's CDR2 significantly reduced binding affinity for RTA (~10-fold weaker; 1.76 nM versus 0.102 nM) and eliminated toxin-neutralizing activity (Rudolph et al., 2018; PMID: 30265352).
AB03296-23.159 Datasheet
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