Anti-Ricin [F8(JOB10)]
Catalogue Number: AB03297-1.159-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | rRNA N-glycosidase; Ricin A chain (EC:3.2.2.22); Ricin B chain |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Mouse |
| Clone: | F8(JOB10) |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing alpacas, followed by library construction and panning on RTA. |
| Application: | ELISA, NT, Crstapy |
Additional Text
Purification
Purified
Gene ID
8261245
Gene Name
LOC8261245
Uniprot ID
P02879
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The crystal structure of the VHH antibody in complex with the ricin toxin's enzymatic subunit (RTA) was solved. The specificity of the original format of the antibody was confirmed by ELISA analysis, and the antibody's EC50 for ricin holotoxin was 0.5-0.8 nM. The binding affinity of the VHH fragments to ricin was measured by surface plasmon resonance (Kd= 0.2 nM). In the Vero cell cytotoxicity assay, the VHH had an IC50 of ~300 nM, thereby classifying it as a weak neutralizing antibody (Rudolph et al., 2016; PMID: 27159829).
AB03297-1.159 Datasheet
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