Anti-RNA hairpin [BL3-6S97N]
Catalogue Number: AB03300-1.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | BL3-6S97N |
| Isotype: | IgG1 |
| Immunogen: | A library of Fabs was generated by rationally varying the composition of the residues in complementarity determining regions (CDRs) of Fab BL3-6. Subsequently, the Fab library was challenged in a phage display selection to recognize and bind the GAAACAC motif. |
| Application: | Crstion |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The Fab was generated by directed mutagenesis from the parental Fab BL3-6. The S97N mutation in the complementarity determining region L3 of the Fab improved the binding affinity compared to the parent Fab BL3-6 (Kd = 5 nM). The Fab fragment was also found to bind the affinity-matured GAGACCC hairpin. The crystal structure of the Spinach RNA grafted with GAAACAC loop in complex with Fab BL3-6S97N was determined (Koirala et al., 2018; PMID: 29309709).
Short Description
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse Fab format for improved compatibility with existing reagents assays and techniques.
AB03300-1.1-BT Datasheet
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