Anti-HLA-DR [L243]
Catalogue Number: AB03368-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD74; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | L243 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing mice with the human B cell line RPMI 8866. |
| Application: | ELISA, FC, ICC, IF, IP, WB, IHC, Blk, InVivoA |
Additional Text
Gene ID
972
Gene Name
CD74
Uniprot ID
P04233
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2 format for improved compatibility with existing reagents assays and techniques.
Application Notes
This antibody can be used for the immunoprecipitation of HLA-DR and MHC class-II peptides (Bonnefoy et al., 1988; PMID: 2961843). This antibody selectively inhibited the in vitro proliferation of Epstein-Barr virus-transformed lymphoblastoid cell lines (Kabelitz and Janssen, 1989; PMID: 2467753). This antibody can also be used for the immunocytochemical and immunohistochemical analysis of frozen sections of necropsy brain tissue to identify HLA-DR-expressing cells (Esiri et al., 1991; PMID: 1864982). This antibody was used in the detection of MHC class II protein in a western blot (Esser et al., 2001; PMID: 11390619). This antibody was used in a flow cytometric assay to detect the decrease in HLA-DR expression on monocytes, which is an indicator of immunosuppression in patients with sepsis and is correlated with an increased risk of secondary infection and mortality (Quadrini et al., 2021; PMID: 33432735). Alexa 568- and FITC-labeled versions of this antibody were used in the immunofluorescent staining of renal microvascular endothelial cells (RMEC) or human kidney tissue (Muczynski et al., 2003; PMID: 12707403). This antibody was assessed in a pilot study using a canine lymphoma model. In vitro studies showed that this antibody binds to both healthy and lymphoma canine lymphocytes, inducing apoptosis in canine non-Hodgkin lymphoma cells. In vivo studies confirmed the antibody's safety in healthy and lymphoma-affected dogs, as well as its binding activity in lymphoma-affected lymph nodes. Preliminary results indicated transient disease stabilization in a subset of dogs with advanced lymphoma (Stein et al., 2011; PMID: 21133722).
AB03368-23.0-BT Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations