Anti-CD45 [BC8]
Catalogue Number: AB03391-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Receptor-type tyrosine-protein phosphatase C; L-CA; Leukocyte common antigen; T200; Lymphocyte antigen 5 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | BC8 |
| Isotype: | IgG |
| Immunogen: | |
| Application: | FC, InVivoA |
Additional Text
Gene Name
PTPRC
Gene ID
5788
Uniprot ID
P08575
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The antibody was used to target human Ramos lymphoma xenografts in athymic mice. The antibody was able to deliver radioiodine to tumors, causing minimal toxicity to normal organs. Further, therapy experiments demonstrated that 90Y-(DOTA)-biotin with BC8-SA cured the mice in a dose dependent manner, and with complete remission of the antibody (Pagel et al., 2003; PMID: 12446461). In vivo studies on Macaca nemestrine revealed the 131l-labeled IgG1 antibody could deliver radiation with relative specificity to lymphohematopoietic tissues. The antibody displayed an association constant (avidity) of 6 x 10^7 (L/mol) for macaque cells and 5 x 10^8 (L/mol) for human cells (Matthews et al., 1991; PMID: 1832994). Furter, 211At labeled BC8-B10 is of interest for clinical trials involving allogeneic hematopoietic transplantation in the treatment of advanced hematological malignancies (Li et al., 2018; PMID: 30335787). 125I-BC8 was stably retained on the surface of lymphoma cells without appreciable endocytosis or shedding (Press et al., 1994; PMID: 8118040). 90Y-BC8-DOTA in combination with Fludarabine and TBI entered phase I Trial as conditioning for allogeneic peripheral blood stem cell transplant (Tuazon et al., 2017). The humanized version of the antibody was constructed. This antibody was used for detection of cytotrol cells by flow cytometry. The antibody was radiolabeled with actinium-225 (225AC) or lutetium-177 (177Lu), and used as therapeutic for the treatment of malignant and non-malignant hematological diseases and disorders (WO2021055638).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
AB03391-23.0-BT Datasheet
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