Anti-envelope protein [ZIKVEF2L01]

Catalogue Number: AB03413-30.11-BT-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:ZIKV envelope protein; E protein
Shipping Condition:Blue Ice
Unit(s): 500 ug
Host name:
Clone: ZIKVEF2L01
Isotype:
Immunogen: The original antibody was generated by immunizing chicken with ZIKV envelope protein followed by antibody library construction and phage display-based selection.
Application: ELISA, FC, WB, InVitroInh

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

The specificity of the scFv format of the antibody was confirmed by ELISA analysis. The antibody detected the recombinant E protein by western blot analysis. The scFv format recognized the E protein in a transfected Vero cell by flow cytometry. Competitive inhibition assay was employed to calculate the percentage of inhibition and the dissociation constant of the antibody against the E protein (inhibition of 50% = 0.55 ug/mL and Kd= 2.04 *10^-8 M). Therefore, the antibody has diagnostic or therapeutic potential for ZIKV (PMID: 31940993).