Anti-envelope protein [ZIKVEF3S06]
Catalogue Number: AB03414-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | ZIKV envelope protein; E protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.2 mg |
| Host name: | Rabbit |
| Clone: | ZIKVEF3S06 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing chicken with ZIKV envelope protein followed by antibody library construction and phage display-based selection. |
| Application: | ELISA, FC, WB, InVitroInh |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The specificity of the scFv format of the antibody was confirmed by ELISA analysis. The antibody detected the recombinant E protein by western blot analysis. The scFv format recognized the E protein in a transfected Vero cell by flow cytometry. Competitive inhibition assay was employed to calculate the percentage of inhibition and the dissociation constant of the antibody against the E protein (inhibition of 50% = 77.42 ug/mL and Kd= 4.55 *10^-6 M).The antibody has diagnostic or therapeutic potential for ZIKV (PMID: 31940993).
Short Description
This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format for improved compatibility with existing reagents assays and techniques.
AB03414-23.0 Datasheet
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