Anti-Hexon [9C12]
Catalogue Number: AB03440-1.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Hexon protein; CP-H; Protein II |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | 9C12 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing mice with Ad type 5 (Ad5). |
| Application: | ELISA, IF, IP, EM, WB, NT, Blk, SPR, Crstion |
Additional Text
Purification
Purified
Uniprot ID
P04133
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The antibody was able to neutralize Ad5. Cryo-EM reconstruction of the Ad5-9C12 complex showed that the capsid is coated by a meshwork of bivalently bound antibody molecules. The antibody bound to the hexon protein of Ad5 particles denatured as shown by western blot analysis (Varghese et al., 2004; PMID: 15507619).The crystal structure of scFv fragment of the antibody in complex with human adenovirus C5 hexon was determined (Schmid et al., 2018; PMID: 29386504). The specificity of the original format of the antibody was confirmed by ELISA analysis (Moyer et al., 2016; PMID: 26491163). Wild-type viruses were immunoprecipitated using the antibody (Myerset al., 2013; PMID: 23487468). The binding affinity of the Fab fragment to Adv5 hexon was measured by surface plasmon resonance (Kd= 1 nM) (Bottermann et al., 2016; PMID: 27881870). The antibody detected hexon on A549 cells by immunofluorescence analysis (Smith et al., 2008; PMID: 18448546).
AB03440-1.1-BT Datasheet
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