Anti-Arg-2 [CPR02]
Catalogue Number: AB03441-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Human Anterior Gradient Protein 2 Homolog; Anterior gradient protein 2 homolog; AG-2; hAG-2; HPC8; Secreted cement gland protein XAG-2 homolog |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | CPR02 |
| Isotype: | IgG |
| Immunogen: | The antibody was isolated from the antibody library after three rounds of panning against residues 41-175 of recombinant human Agr2. |
| Application: | ELISA, IF, InVivoA, SPR |
Additional Text
Gene Name
AGR2
Gene ID
10551
Uniprot ID
O95994
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The scFv fragment of the antibody was able to bind the recombinant human Agr2 protein in ELISA analysis. The binding affinity of the scFv fragment to human Agr2 was measured by surface plasmon resonance (Kd= 4.6x10^-7 M). Immunofluorescence analysis of colon biopsy samples from a healthy donor was performed to verify the ability of CPR02 to recognize the human Agr2 antigen. Results showed the antibody revealed significant staining surrounding the crypts of the intestinal tissue. Further, the antibody was used for immunofluorescent staining of freshly frozen human biopsy samples of Ulcerative Colitis, the antibody produced a strong intensity signal in the colonic crypts region. The antibody was able to recognise the Agr2 antigen in vivo, as demonstrated by the results of the autoradiography of the complete intestinal tract of a mouse having colitis, injected with radiolabelled antibody (US20190309060A1).
Short Description
This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.
AB03441-23.0-BT Datasheet
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