Anti-spike protein [TZZ14]
Catalogue Number: AB03446-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | TGEV; Spike glycoprotein; S glycoprotein; E2; Peplomer protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Rabbit |
| Clone: | TZZ14 |
| Isotype: | IgG |
| Immunogen: | A scFv antibody phage display library was constructed from peripheral blood lymphocytes of piglets infected with TGEV. The library was screened with four rounds of biopanning using purifed TGEV antigen, and scFv antibodies that bound to TGEV were obtained. |
| Application: | ELISA, IF, WB, InVitroA |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Uniprot ID
P07946
Application Notes
To determine whether scFv antibody neutralized TGEV infection, a PRN assay was performed. The results showed that the antibody signifcantly inhibited TGEV infection in a dose-dependent manner and completely neutralized virus replication at a dilution of 1:40. Therefore the antibody showed a neutralization efect on TGEV infection. The specificity of the scFv format of the antibody was confirmed by ELISA analysis. The antibody detected the S protein by western blot analysis. IFA results showed that the antibody bound to ST TGEV-infected cells and to TGEV S protein-transfected cells (Zhang et al., 2019; PMID: 30729994).
Short Description
This full-length chimeric rabbit antibody was made using the variable domain sequences of the original scFv format for improved compatibility with existing reagents assays and techniques.
AB03446-23.0 Datasheet
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