Anti-Clusterin [16B5]

Catalogue Number: AB03504-10.3-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	CLU; APOJ; CLI; KUB1; AAG4
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Human
Clone:	16B5
Isotype:	IgG1
Immunogen:
Application:	IF, Inh, InVitroA, InVivoA

Overview
Datasheets

Additional Text

Uniprot ID

P10909

Gene Name

CLU

Gene ID

1191

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

The effect of the original format of the antibody was evaluated on lung metastasis in BALB/c mice with orthotopically implanted 4T1 cells. The antibody was able to significantly reduce lung metastasis (Lenferink et al., 2010; PMID: 19935703). A humanized version of the antibody was constructed. The humanized version of the antibody inhibited the EMT-inducing activity of clusterin on A549 NSCLC cells. Immunofluorescence was performed on A549 cells using this antibody to monitor E-cadherin expression. The combination of the humanized version of the antibody with erlotinib resulted in an enhanced anti-tumour effect (H1299 and H460 cells) (US9822170). AB-16B5 is a humanized IgG2 version of the antibody which specifically binds to tumour-associated clusterin and inhibits its activity. This antibody showed a good tumour inhibitory effect in in vivo application (Tremblay et al.; 2010). A phase I and phase II trial to assess safety and tolerability of AB-16B5 for the treatment of solid tumours was performed (Ferrario et al., 2017; NCT04364620).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

AB03504-10.3-BT Datasheet

Price

£1,825.00

-

Quantity
Unit Size

Find out about delivery