Anti-LAG-3 [B5D1]
Catalogue Number: AB03511-10.3-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD223; LAG3; FDC; Lymphocyte activation gene 3 protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | B5D1 |
| Isotype: | IgG1 |
| Immunogen: | |
| Application: | ELISA, FC, Blk, InVitroA, SPR |
Additional Text
Uniprot ID
P18629
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Gene ID
1113363; 1113371
Gene Name
repR
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The humanized version of the antibody was constructed. The specificity of the original format of the antibody was confirmed by ELISA analysis. The binding activity of the humanized antibody to LAG3 was EC 50= 63.3 ng/ml. The binding affinity of the humanized antibody was measured by surface plasmon resonance (Kd= 6.10^-10 M). This antibody was used for detection of LAG-3 expressed on CHO cells by flow cytometry. The antibody blocked the binding of LAG-3 antigen and MHC-II molecule (IC50= 0.152 ug/ml). The antibody could effectively activate the activity of antigen-specific T cells, EC50 of in vitro activity test results of B5D1 antibody equal to 1.25 ug/ml (CN110606892).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
AB03511-10.3-BT Datasheet
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