Anti-non-structural protein 4 [Nb43]
Catalogue Number: AB03514-1.159-BS-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | NSP4; Replicase polyprotein 1ab; ORF1ab polyprotein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | Nb43 |
| Isotype: | IgG1 |
| Immunogen: | An adult male Bactrian camel was immunized subcutaneously with the purified Nsp4-NHis recombinant protein. A camel VHH library was then constructed and panned against Nsp4. |
| Application: | ELISA, InVitroA |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The specificity of the original format of the antibody was confirmed by indirect ELISA analysis. MARC-145 cell lines expressing nanobodies were generated and infected with PRRSV strain SD16. At 24 h post-infection the antibody inhibited infectious virus release by about 99%. Further, the antibody protected MARC-145 cells from any virus-induced cytopathic effect and fully blocked PRRSV replication (Liu et al. 2016; PMID: 27010387).
AB03514-1.159-BS Datasheet
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