Anti-VACV [SC212]
Catalogue Number: AB03517-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | VV |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | SC212 |
| Isotype: | IgG |
| Immunogen: | A phage display library of human single chain variable fragment (scFv) antibodies was constructed from the B cells of healthy volunteers boosted by the VACV vaccine. Human scFv was isolated by panning against VACV. |
| Application: | ELISA, InVitroNT |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The scFv fragment was converted to human IgG1 (VC212). The binding affinity of VC212 was estimated by competition ELISA to IC50 values 22 µg/ml (146.67 nM). The humanized version of the antibody neutralized the VACV, as evidenced by the plaque reduction neutralization test result with a PRNT50 of ~0.16 mg/ml (~1.07 µM) (Shin et al., 2019; PMID: 30856707).
Short Description
This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.
AB03517-23.0 Datasheet
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