Anti-S protein (RBD) [aRBD-5]

Catalogue Number: AB03532-1.159-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	0.5 mg
Host name:	Mouse
Clone:	aRBD-5
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing two alpacas with highly purified recombinant SARS-CoV-2 RBD, and developing a VHH library. The antibody was isolated by panning against SARS-CoV-2 RBD.
Application:	ELISA, NT, Blk, SPR

Overview
Datasheets

Additional Text

Gene Name

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Gene ID

43740568

Uniprot ID

P0DTC2

Specificity

SARS CoV 2 S glycoprotein; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV; SARS-CoV2

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

The VHH and VHH with IgG1 Fc formats demonstrated strong binding to both RBD and the entire ectodomain (S1+S2) of SARS-CoV-2 spike in ELISA, with a low nanomolar 50% effective concentration (EC50 respectively 0.674 nM and 1.016 nM). The binding affinity of the VHH format and VHH-Fc formats to RBD were also measured using surface plasmon resonance, with KD values of 16.3 nM and 1.25 nM respectively. Both VHH and Fc fusion formats could block RBD-ACE2 interaction in a dose-dependent manner, as characterized by competitive ELISA. Furthermore, the fusion of two VHHs with nonoverlapping epitopes resulted in a hetero-bivalent VHHs, aRBD-2-5, which showed significantly higher RBD binding affinities (KD of 59.2 pM). Similarly, the Fc fusion also showed enhanced binding affinities, with KD values of 12.3 pM. The homo- and hetero-bivalent VHHs exhibited potent neutralizing ability against SARSCoV-2 inoculated onto Vero E6 cells. The 50% neutralization dose was 1.22 ng/ml (0.043 nM) for aRBD-2-5. Finally, the ND50s for aRBD-2-5-Fc was 11.8 ng/ml (0.107 nM) (Ma et al, 2021; PMID:33658349).

AB03532-1.159-BT Datasheet

Price

£2,215.00

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