Anti-pre-fusion viral F protein [MPE8]

Catalogue Number: AB03555-1.1-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Human respiratory syncytial virus; HRSV; human metapneumovirus; HMPV; Fusion glycoprotein F0; Protein F; PVM; BRSV
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Mouse
Clone:	MPE8
Isotype:	IgG1
Immunogen:	The original antibody was generated by isolating IgG1 memory B cells of a blood donor with high serum neutralizing antibody titres against HRSV and HMPV.
Application:	ELISA, WB, NT, Inh, InVivoA, SPR, Crstion

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Gene Name

Uniprot ID

P03420, Q6WB98

Gene ID

2799939

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

The antibody neutralized HRSV and HMPV, as well as PVM and BRSV, with IC50 (half-maximum inhibitory concentration) values ranging from 2 to 110 ng/ml. The antibody was also tested for its capacity to prevent cell-to-cell viral spread, showing a 50% spreading inhibiting capacity (IS50). The antibody selectively bound to the pre-fusion F protein, as shown by SPR. The antibody detected HRSV and HMPV F proteins under non-reducing conditions in western blot analysis. Further, the antibody showed prophylactic and therapeutic efficacy in vivo. In the HRSV mouse model, the antibody could reduce HRSV lung titres and was effective at concentrations as low as 0.12 mg per kg of body weight. Similar results were obtained in the HMPV mouse mode. The antibody fully protected mice from PVM lethality at 0.12 mg per kg and from body weight loss at 0.6 mg per kg. Moreover, in a therapeutic setting the antibody completely protected from lethality when administered up to 3 days after infection both at 30 and 5 mg per kg and conferred significant protection when given on day 4 or 5 at 30 mg per kg (Corti et al., 2013; PMID: 23955151). The structure of the scFv fragment in complex with the F protein of RSV was solved (Wen et al., 2017; PMID: 28134915). In ELISA analysis, the antibody bound to pre-fusion and uncleaved postfusion F, albeit with lower affinity (Gilman et al., 2015; PMID: 26161532).

Short Description

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB03555-1.1-BT Datasheet

Price

£1,825.00

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