Anti-GFP [Minimizer]
Catalogue Number: AB03618-1.159-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Green fluorescent protein; MI; GBP4 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Mouse |
| Clone: | Minimizer |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing camels with GFP. A phage display library was constructed and the antibody was selected by panning against GFP. |
| Application: | InVitroA, Crstion |
Additional Text
Uniprot ID
P42212
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The antibody could reversibly reduce GFP fluorescence by a factor of 5 in vitro. A comparable fluorescence modulation was also observed after addition of the antibody to soluble cell extract derived from human embryonic kidney (HEK) 293T cells expressing eGFP. The crystal structure of the GFP-antibody complex was determined. The dissociation constant of the antibody was measured (Kd= 0.45 nM) (Kirchhofer et al., 2010; PMID: 20010839). Fluorescence modulation in living cells was also observed after the addition of the antibody to HeLa cells expressing GFP (Schmidthals et al., 2010; PMID: 20372881).
AB03618-1.159 Datasheet
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