Anti-PD-L1 [BT613]
Catalogue Number: AB03784-10.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD274; B7-H1; B7 homolog 1; PDCD1 ligand 1; Programmed death ligand 1; hPD-L1; Programmed cell death 1 ligand 1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Human |
| Clone: | BT613 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing transgenic mice with PD-L1, followed by antibody library construction and phage display-based selection against PD-L1. |
| Application: | Blk, Inh, InVitroA, InVivoA, SPR |
Additional Text
Gene Name
CD274
Gene ID
29126
Uniprot ID
Q9NZQ7
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The binding affinity of the antibody to PD-L1 was measured by surface plasmon resonance. The binding affinity of the antibody was measured on the cell lines with high expression of PD-L1 by FACS. The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody had good activity in blocking the binding of PD-L1 to PD-1 and PD-L1 to B7-1 is shown by ELISA analysis. The antibody could block the binding of PD-1 and PD-L1 at the cellular level in in vitro experiments. The antibody was used for in vivo experiments on colon cancer tumor model. B-hPD-1 mice with human PD-1 and MC38-hPD-L1 colon cancer cells with human PD-L1 were treated with the antibody. Results showed inhibition of tumor volume growth in mice treated with the antibody.
AB03784-10.0 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations