Anti-PD-L1 [BT613]
Catalogue Number: AB03784-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD274; B7-H1; B7 homolog 1; PDCD1 ligand 1; Programmed death ligand 1; hPD-L1; Programmed cell death 1 ligand 1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Rabbit |
| Clone: | BT613 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing transgenic mice with PD-L1, followed by antibody library construction and phage display-based selection against PD-L1. |
| Application: | Blk, Inh, InVitroA, InVivoA, SPR |
Additional Text
Gene Name
CD274
Gene ID
29126
Uniprot ID
Q9NZQ7
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The binding affinity of the antibody to PD-L1 was measured by surface plasmon resonance. The binding affinity of the antibody was measured on the cell lines with high expression of PD-L1 by FACS. The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody had good activity in blocking the binding of PD-L1 to PD-1 and PD-L1 to B7-1 is shown by ELISA analysis. The antibody could block the binding of PD-1 and PD-L1 at the cellular level in in vitro experiments. The antibody was used for in vivo experiments on colon cancer tumor model. B-hPD-1 mice with human PD-1 and MC38-hPD-L1 colon cancer cells with human PD-L1 were treated with the antibody. Results showed inhibition of tumor volume growth in mice treated with the antibody.
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB03784-23.0 Datasheet
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