Anti-Flavivirus group antigen [2A10G6]
Catalogue Number: AB03862-10.3-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Genome polyprotein; Envelope protein E; CGMCC No. 3292 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Human |
| Clone: | 2A10G6 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with neonatal rat brain suspension infected with Dengue 2 virus. |
| Application: | ELISA, IF, NT, InVitroA, InVivoA, Crstapy |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Gene ID
1502173
Gene Name
POLY
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
UniProt ID
P03314, Q99D35
Application Notes
The specificity of the antibody for the E protein was confirmed by ELISA analysis. The antibody recognized dengue virus prM-E antigen by indirect immunofluorescence. Hamster kidney cell line BHK-21 was used to verify the inhibitory effect of the antibody on multiple flavivirus infections. The results of cell neutralization experiments showed that antibody can effectively block a variety of flavivirus infections and the binding of BHK21 cells. In vivo protection experiments showed that antibodies against flavivirus envelope E protein can effectively protect suckling mice against dengue virus attack, and the survival rate and survival time are significantly improved (CN101891806B). The crystal structure of the Fab fragment of the antibody in complex with zika virus envelope protein was determined. The antibody bound to the ZIKV-E protein with a high binding affinity (Kd= 2.7 nM) and neutralized the ZIKV infection in vitro. Further, the antibody completely protects mice against the circulating ZIKV strain in vivo (Dai et al., 2016; PMID: 27158114).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB03862-10.3 Datasheet
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