Anti-HPV16 E7 protein [69E2]

Catalogue Number: AB03909-10.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	E7; Protein E7; Human papillomavirus type 16 E7 protein
Shipping Condition:	Blue Ice
Unit(s):	0.1 mg
Host name:	Human
Clone:	69E2
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing BALB/c mice with recombinant HPV16 E7 protein.
Application:	ELISA, ICC, IF, WB, IHC, CLIA

Overview
Datasheets

Additional Text

Gene ID

1489079

Gene Name

Uniprot ID

P03129

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The binding characterization of this antibody for HPV E7 protein was done using ELISA. The original IgG2a version of this antibody binds HPV E7 protein with a binding affinity of Kd= 5.6 nM. The anti-HPVE7 protein antibody 79A11 can be used as capture antibody in combination with HRP conjugated 69E2 antibody as a detection antibody in a sandwich ELISA. This antibody can be used for the detection of HPV E7 protein from cells lysates of cervical cancer cell lines CaSki cells and HeLa cells in a western blot. This antibody was also used in the immunocytochemical analysis of CaSki cells and HeLa cells (HPV positive). This antibody was used for the immunohistochemical analysis of HPV16-positive cervical cancer squamous cell carcinoma and HPV18-positive cervical cancer adenocarcinoma. This antibody was also used for the indirect immunofluorescence staining of CaSki cells and HeLa cells. A chemiluminescent immunoassay was prepared using 79A11 as capture and Biotin-69E2 and HRP-Streptavidin for detection (CN111410689).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

AB03909-10.0 Datasheet

Price

£430.00

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