Anti-HPV16 E7 protein [69E2]

Catalogue Number: AB03909-2.3-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	E7; Protein E7; Human papillomavirus type 16 E7 protein
Shipping Condition:	Blue Ice
Unit(s):	0.1 mg
Host name:	Mouse
Clone:	69E2
Isotype:	IgG2a
Immunogen:	The original antibody was generated by immunizing BALB/c mice with recombinant HPV16 E7 protein.
Application:	ELISA, ICC, IF, WB, IHC, CLIA

Overview
Datasheets

Additional Text

Gene ID

1489079

Gene Name

Uniprot ID

P03129

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The binding characterization of this antibody for HPV E7 protein was done using ELISA. The original IgG2a version of this antibody binds HPV E7 protein with a binding affinity of Kd= 5.6 nM. The anti-HPVE7 protein antibody 79A11 can be used as capture antibody in combination with HRP conjugated 69E2 antibody as a detection antibody in a sandwich ELISA. This antibody can be used for the detection of HPV E7 protein from cells lysates of cervical cancer cell lines CaSki cells and HeLa cells in a western blot. This antibody was also used in the immunocytochemical analysis of CaSki cells and HeLa cells (HPV positive). This antibody was used for the immunohistochemical analysis of HPV16-positive cervical cancer squamous cell carcinoma and HPV18-positive cervical cancer adenocarcinoma. This antibody was also used for the indirect immunofluorescence staining of CaSki cells and HeLa cells. A chemiluminescent immunoassay was prepared using 79A11 as capture and Biotin-69E2 and HRP-Streptavidin for detection (CN111410689).

Short Description

This is a reformatted mouse IgG2a Fc Silent™ antibody, based on the original mouse IgG2a format, created for improved compatibility with existing reagents, assays and techniques.

AB03909-2.3 Datasheet

Price

£430.00

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