Anti-Vimentin [YT33]

Catalogue Number: AB03911-23.0-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Rabbit
Clone:	YT33
Isotype:	IgG
Immunogen:	The original antibody was generated by immunizing BDF1 mice with keyhole limpet hemocyanin (KLH) coupled PV33 - a synthesized vimentin peptide containing the ser33 phosphorylation - (PV33-KLH) emulsified in complete Freund's adjuvant.
Application:	ELISA, ICC, IF, IHC-P, WB, IEM

Overview
Datasheets

Additional Text

Gene Name

VIM

Uniprot ID

P31000

Gene ID

81818

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody is specific to the C kinase phosphorylated vimentin Ser33 residue, as determined by ELISA, can detect the activity of endogenous CAM kinase II astrocytes, as detected by WB, and was used for ICC (IF) to detect C kinase phosphorylated vimentin Ser33 in type 1 astrocytes extracted from newborn rats' cerebral cortices (Ogawara et al., 1995; PMID: 7490282). This antibody was used for: in vivo vimentin kinase activity visualization of vimentin kinase activity in cell signaling or mitosis (Goto et al., 1998; PMID: 9565595), IHC-p and IF staining of human glioma cells (Fujita et al., 2004; PMID: 15597762), and as a positive control in a WB for vimentin ser55 phosphorylation (Yamaguchi et al., 2005; PMID: 16260496).

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB03911-23.0-BT Datasheet

Price

£1,825.00

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