Anti-Vimentin [MO82]

Catalogue Number: AB03912-10.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	0.1 mg
Host name:	Human
Clone:	MO82
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing BDF1 mice with keyhole limpet hemocyanin (KLH) coupled PV82 - a synthesized vimentin peptide containing the ser82 phosphorylation - (PV82-KLH) emulsified in complete Freund's adjuvant.
Application:	ELISA, ICC, IF, WB, IEM

Overview
Datasheets

Additional Text

Gene Name

VIM

Uniprot ID

P31000

Gene ID

81818

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody is specific to the CAM kinase II phosphorylated vimentin Ser82 residue, as determined by ELISA, can detect the activity of endogenous CAM kinase II astrocytes, as detected by WB, and was used for ICC (IF) to detect CAM kinase II phosphorylated vimentin Ser82 in type 1 astrocytes extracted from newborn rats' cerebral cortices. IEM observation confirmed that the epitope of MO82 was localized along the vimentin filaments (Ogawara et al., 1995; PMID: 7490282). This antibody was used for: in vivo vimentin kinase activity visualization of vimentin kinase activity in cell signaling or mitosis (Goto et al., 1998; PMID: 9565595) and as a positive control in a WB for vimentin ser82 phosphorylation and in IF staining of U251 human glioma cells (Yamaguchi et al., 2005; PMID: 16260496).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB03912-10.0 Datasheet

Price

£430.00

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