Anti-HPV16 E7 protein [79A11]
Catalogue Number: AB03950-21.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | E7; Protein E7; Human papillomavirus type 16 E7 protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.05 mg |
| Host name: | Mouse |
| Clone: | 79A11 |
| Isotype: | IgM |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with recombinant HPV16 E7 protein. |
| Application: | ELISA, ICC, IF, WB, IHC, CLIA |
Additional Text
Gene ID
1489079
Gene Name
E7
Uniprot ID
P03129
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The binding characterization of this antibody for HPV E7 protein was done using ELISA. This antibody can be used as a capture antibody in combination with HRP conjugated anti-HPVE7 protein 69E2 antibody as a detection antibody in a sandwich ELISA. This antibody can be used for the detection of HPV E7 protein from cells lysates of cervical cancer cell lines CaSki cells and HeLa cells in a western blot. This antibody was also used in the immunocytochemical analysis of CaSki cells and HeLa cells (HPV positive). This antibody was used for the immunohistochemical analysis of HPV16-positive cervical cancer squamous cell carcinoma and HPV18-positive cervical cancer adenocarcinoma. This antibody was also used for the indirect immunofluorescence staining of CaSki cells and HeLa cells. A chemiluminescent immunoassay was prepared using 79A11 as capture and Biotin-69E2 and HRP-Streptavidin for detection (CN111333720).
AB03950-21.0 Datasheet
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