Anti-HPV16 E7 protein [79A11]
Catalogue Number: AB03950-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | E7; Protein E7; Human papillomavirus type 16 E7 protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Rabbit |
| Clone: | 79A11 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with recombinant HPV16 E7 protein. |
| Application: | ELISA, ICC, IF, WB, IHC, CLIA |
Additional Text
Gene ID
1489079
Gene Name
E7
Uniprot ID
P03129
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The binding characterization of this antibody for HPV E7 protein was done using ELISA. This antibody can be used as a capture antibody in combination with HRP conjugated anti-HPVE7 protein 69E2 antibody as a detection antibody in a sandwich ELISA. This antibody can be used for the detection of HPV E7 protein from cells lysates of cervical cancer cell lines CaSki cells and HeLa cells in a western blot. This antibody was also used in the immunocytochemical analysis of CaSki cells and HeLa cells (HPV positive). This antibody was used for the immunohistochemical analysis of HPV16-positive cervical cancer squamous cell carcinoma and HPV18-positive cervical cancer adenocarcinoma. This antibody was also used for the indirect immunofluorescence staining of CaSki cells and HeLa cells. A chemiluminescent immunoassay was prepared using 79A11 as capture and Biotin-69E2 and HRP-Streptavidin for detection (CN111333720).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
AB03950-23.0 Datasheet
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