Anti-AIF1 [GT1-mAb1]

Catalogue Number: AB03986-24.1-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	AIF1; IBA1; allograft inflammatory factor 1; AIF-1; IRT-1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; Daintain; DADB-70P7.8; allograft inflammatory factor-1 splice variant Hara-1; IRT1; protein G1
Shipping Condition:	Blue Ice
Unit(s):	0.1 mg
Host name:	Goat
Clone:	GT1-mAb1
Isotype:	IgG
Immunogen:	This antibody was obtained by sequencing a polyclonal goat IgG raised against a peptide with the sequence C-TGPPAKKAISELP.
Application:	ELISA, FC, IF, WB

Overview
Datasheets

Additional Text

Gene ID

199

Uniprot ID

P55008

Gene Name

AIF1

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody (GT1-mAb1) was one of two monoclonal antibodies discovered by analyzing a polyclonal antibody (GT-1) sample. Briefly, de novo sequencing of GT-1 resulted in the full-length sequences of two distinct monoclonal antibodies (GT1-mAb1 and GT1-mAb2) in both framework and CDR regions. Both antibodies were recombinantly expressed by Absolute Antibody in HEK293 cells and tested in various assays. GT1-mAb1 was tested against the peptide antigen in an ELISA, and it showed activity comparable to that of the original polyclonal antibody. GT1-mAb1 was found to bind the target peptide in a non-competitive manner with GT1-mAb2 while being efficiently blocked by the original polyclonal antibody, proving that both monoclonal antibodies bind distinct epitopes and that GT1-mAb1 possess binding characteristics of the input polyclonal antibody. Furthermore, GT1-mAb1 was compatible with immunofluorescence (IF), flow cytometry (FC), and Western blot (WB), with an FC signal observedly more distinct than that of GT1-mAb2.

AB03986-24.1 Datasheet

Price

£430.00

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