Anti-AIF1 [GT1-mAb2]
Catalogue Number: AB03987-24.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | AIF1; IBA1; allograft inflammatory factor 1; AIF-1; IRT-1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; Daintain; DADB-70P7.8; allograft inflammatory factor-1 splice variant Hara-1; IRT1; protein G1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Goat |
| Clone: | GT1-mAb2 |
| Isotype: | IgG |
| Immunogen: | This antibody was obtained by sequencing a polyclonal goat IgG raised against a peptide with the sequence C-TGPPAKKAISELP. |
| Application: | ELISA, FC, IF |
Additional Text
Gene ID
199
Uniprot ID
P55008
Gene Name
AIF1
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody (GT1-mAb2) was one of two monoclonal antibodies discovered by analyzing a polyclonal antibody (GT-1) sample. Briefly, de novo sequencing of GT-1 resulted in the full-length sequences of two distinct monoclonal antibodies (GT1-mAb1 and GT1-mAb2) in both framework and CDR regions. Both antibodies were recombinantly expressed by Absolute Antibody in HEK293 cells and tested in various assays. GT1-mAb2 was tested against the peptide antigen in an ELISA, and it showed activity comparable to that of the original polyclonal antibody. GT1-mAb2 was found to bind the target peptide in a non-competitive manner with GT1-mAb1 while being efficiently blocked by the original polyclonal antibody, proving that both monoclonal antibodies bind distinct epitopes and that GT1-mAb2 possess binding characteristics of the input polyclonal antibody. Furthermore, GT1-mAb2 was found to be compatible with immunofluorescence (IF) and flow cytometry (FC), although with an FC signal observedly less distinct than that of GT1-mAb1.
AB03987-24.1-BT Datasheet
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