Anti-CD3 [CA17.2A12]

Catalogue Number: AB03988-23.0-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	TCR/CD3; TCR-2; TCR-αβ; TCR-ab; TCR-alphabeta
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Rabbit
Clone:	CA17.2A12
Isotype:	IgG
Immunogen:	The original antibody was generated by immunizing mice with the T cell receptor complex (TCR/CD3) isolated from canine thymocytes.
Application:	FC, IHC, Act

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody was used as an immune phenotype marker in flow cytometric (FC) analyses of peripheral blood lymphocytes (PBL) in dogs to identify T cells (specifically, CD3) (Helfand et al., 1995; PMID: 7605993). Later, it was used for immunophenotyping by FC to identify T-cell antigen CD3 in dogs with lymphocytosis (McDonough et al., 2000; PMID: 11105953) and as a T-lymphocyte marker in blood and bone marrow samples from dogs with hematologic disorders (Weiss, 2001; PMID: 11817066). It was also used in FC analysis of various cell populations, including peripheral blood and lymph nodes, to identify CD3+ T cells in a study on apoptosis (Pappalardoa et al., 2001; PMID: 11730925). Most recently, it was used to stain leukocytes for analysis by FC as part of a Canine leukocyte suppression assay (LSA) (Amorim et al., 2020; PMID: 32698861), to identify CD3 in fine-needle aspirates of canine nodal lymphomas (Rigillo et al., 2021; PMID: 33759339), and to stain CD3 on canine peripheral blood mononuclear cells (PBMC) (Ruiz et al., 2022; PMID: 36217551). Additionally, this antibody was used to detect CD3 in immunohistology studies (specifically IHC) of the liver and spleen in dogs treated with rcGM-CSF (Nash et al., 1995; PMID: 7655007). This antibody was fixed to magnetic beads and used for the activation of canine peripheral blood lymphocytes (Szopa et al., 2021; PMID: 33679741). If you are interested in canine bispecific T-cell engagers, please contact us.

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB03988-23.0-BT Datasheet

Price

£1,825.00

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