Anti-Occludin [1-3]
Catalogue Number: AB04036-3.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | OCLN |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Mouse |
| Clone: | 1-3 |
| Isotype: | IgG2b |
| Immunogen: | The original antibody was generated by immunizing Wistar rats with a mammalian expression vector encoding hOCLN. |
| Application: | ELISA, FC, ICC, IP, Blk, Inh, InVitroA, InVivoA |
Additional Text
Gene Name
OCLN
Uniprot ID
Q16625
Gene ID
100506658
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The cell binding capability of the original format of this antibody (rat IgG2b) was assessed using FC, and its binding to HT1080/hOCLN cells was assessed using a cell-based ELISA. This antibody was found to have no negative effect on tight junction (TJ) barrier function, as determined by a TJ barrier function assay (TER). Furthermore, this antibody was found to have no negative effect on cell viability, as determined by a colorimetric XTT assay. This antibody was found to strongly inhibit (in vitro) and fully block (in vivo) HCV infections, as measured by ICC (Shimizu et al., 2018; PMID: 29437969). This antibody's Kd for hOCLN is 0.580 +/- 0.031 nM, as measured in a cell-based competition ELISA. This antibody successfully precipitated FLAG-hOCLN proteins found in HT1080 cell lysates and endogenously expressed cellular OCLN found in Caco-2, HepG2, and MCF-7 cell lysates. Furthermore, other TJ proteins expressed in T84 cells - TRIC and ZO-2 - were found to be co-immunoprecipitated with OCLN by this antibody. Immunofluorescence (IF) staining of OCLN using clone 1-3 with a TJ marker protein ZO-1 in Caco-2 cells was co-localized with ZO-1 at cell-cell contact sites. This antibody was found to cross-react with crab-eating monkey (moOCLN), mouse (mOCLN), and rat (rOCLN) as determined by FC (Shimizu et al., 2019; PMID: 31077306).
Short Description
This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2B format, for improved compatibility with existing reagents, assays and techniques.
AB04036-3.0 Datasheet
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