Anti-S. aureus β-GlcNAc WTA [4497]

Catalogue Number: AB04039-10.3-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	TA; Teichoic acids; WTA; Wall teichoic acids; GlcNAc; N-Acetylglucosamine; β-WTA; β-GlcNAc; β-1,4-GlcNAc; β-O-linked N-acetylglucosamine; β-O-linked GlcNAc sugar modifications on WTA
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Human
Clone:	4497
Isotype:	IgG1
Immunogen:	The original antibody was generated by cloning human IgG antibodies from peripheral B cells of patients who had been infected with S. aureus.
Application:	ELISA, FC, IF, InVitroA, InVivoA, RBA

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

The original format of this antibody (human IgG1) was screened for antigen binding specificity using ELISA, and it was successfully used antibody was used in flow cytometry (FC) to label S. aureus (Lehar et al., 2015; PMID: 26536114). This antibody was used in a radioligand binding assay (RBA); affinity analysis and epitope counting were performed by saturation binding analysis using a radio-iodinated version of the antibody (Fong et al., 2018; PMID: 30102105). This antibody was observed to bind strongly to planktonic Wood46 (ATCC 10832) S. aureus cells but very weakly to planktonic LACΔspaΔsbi (AH4116) S. aureus. When studying the binding of WTA-specific antibodies to biofilms, this antibody was observed to bind strongly to PNAG-positive biofilm formed by Wood46 and bind S. aureus Wood46 in both planktonic and biofilm states. Apart from recognizing PNAG-positive biofilms, this antibody was also found to bind the PNAG-negative biofilm formed by LACΔspaΔsbi. Using IF, this antibody was confirmed to bind WTA in PNAG-positive and PNAG-negative in vitro biofilm. This antibody was tested in a subcutaneously implanted pre-colonized catheter in mice and was found to localize to biofilm rapidly in vivo (de Vor et al., 2022; PMID: 34989676).

Short Description

This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.

AB04039-10.3-BT Datasheet

Price

£1,825.00

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