Anti-Cytochrome c [2.7D5]
Catalogue Number: AB04040-3.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CYCS; CYC; HCS; THC4; Cytochrome c, somatic; Cytochrome c; Cyt c |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Mouse |
| Clone: | 2.7D5 |
| Isotype: | IgG2b |
| Immunogen: | The original antibody - a secondary immune response antibody - was generated by immunizing BALB/c mice with pigeon cytochrome C covalently coupled to ovalbumin using glutaraldehyde (CYT-OVA) and later challenging them with the same immunogen (pigeon CYT-OVA). |
| Application: | ELISA, IF, IP, ImGldLbl |
Additional Text
Gene Name
CYCS
Uniprot ID
P62897
Purification
Purified
Gene ID
13063
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The binding specificity of the original format of this antibody (mouse IgG2b) was evaluated by indirect ELISA, and its binding affinity was determined by competitive ELISA. It was found to bind Pigeon CYT ~5.6 times better than Mouse CYT (Liu et al., 2000; PMID: 11257306). This antibody was used in the detection of intracellular mouse cytochrome c (cyt c) in live DO11.10 T hybridoma cells by immunofluorescent (IF) labeling. Specifically, it was found to recognize the surface around residue 62 of cyt c and bound to cyt c in fixed and permeabilized cells. Furthermore, it was used in indirect IF staining of cyt c in post-apoptotic cells and immunoprecipitation (IP) of cyt c extracted from live, apoptotic, and necrotic DO11.10 cells (Jemmerson et al., 1999; PMID: 10090746). This antibody was used in immunogold labeling to determine the subcellular localization of cytochrome-c in rat pancreatic acinar cells (Soltys et al., 2001; PMID: 11319839).
AB04040-3.0 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations